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US 12,360,114 B2Granted
Methods and compositions for protein sequencing
AssigneeQuantum-Si Incorporated
InventorsBrian Reed, Jeremy Lackey, Haidong Huang
Life of this patent
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Abstract
Aspects of the application provide methods of identifying and sequencing proteins, polypeptides, and amino acids, and compositions useful for the same. In some aspects, the application provides methods of obtaining data during a degradation process of a polypeptide, and outputting a sequence representative of the polypeptide. In some aspects, the application provides amino acid recognition molecules comprising a shielding element that enhances photostability in polypeptide sequencing reactions.
Claims22
A method of polypeptide sequencing, the method comprising:
- (a) detecting a first series of signal pulses indicative of association of one or more amino acid recognition molecules with a first amino acid at a terminus of a single polypeptide molecule;
- (b) removing the first amino acid from the terminus of the single polypeptide molecule, wherein a second amino acid is exposed at the terminus of the single polypeptide molecule;
- and
- (c) identifying the second amino acid based at least in part on a first characteristic pattern in the first series of signal pulses, wherein each signal pulse of the first characteristic pattern is indicative of an individual association event between an amino acid recognition molecule and the first amino acid prior to (b).
1 more element
Dependencies are inferred from the claim text.