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US 12,359,243 B2Granted
DNA adductomics by mass tag prelabeling
AssigneeNortheastern University
InventorsRoger W. Giese, Poguang Wang
Life of this patent
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Abstract
Disclosed is a method for detecting the presence of a DNA adduct in DNA. The method involves the pre-labeling of an adducted nucleotide in the DNA with a quaternary ammonium compound.
Claims19
A method for detecting the presence of a DNA adduct in DNA, comprising the following steps:
- (1) reacting an adducted nucleotide in the DNA with a labeling precursor L1 to provide an adducted nucleotide labeled with label L, wherein a nucleophilic moiety on the adducted nucleotide reacts with the labeling precursor L1;
- (2) enzymatically digesting the DNA to provide a free adducted nucleotide comprising the label L;
- and
- (3) detecting the free adducted nucleotide comprising the label L by mass spectrometry,
- wherein the label L comprises a quaternary amine group.
2 more elements
A method for detecting the presence of a DNA adduct in DNA, comprising the following steps:
- (1) reacting an adducted nucleobase in a nucleotide of the DNA with a labeling precursor L1 to provide an adducted nucleobase labeled with label L, wherein a nucleophilic moiety on the adducted nucleobase reacts with the labeling precursor L1;
- (2) subjecting the DNA to neutral thermal hydrolysis to provide a free adducted nucleobase labeled with label L;
- and
- (3) detecting the free adducted nucleobase labeled with label L by mass spectrometry,
- wherein the label L comprises a quaternary amine group.
2 more elements
Dependencies are inferred from the claim text.