Synthetic methylotrophy
AssigneesInstitut National Des Sciences Appliquees De Toulouse, Universite Toulouse III - Paul Sabatier, Centre National De La Recherche Scientifique, Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement
InventorsStéphanie Heux, Alessandro De Simone, Jean-Charles Portais
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Abstract
Disclosed is a microorganism, which has been engineered to acquire methylotrophy. More particularly, the application describes a non-naturally occurring microorganism, which has been engineered to express or include a first enzyme and a second enzyme, wherein the first enzyme is a methanol dehydrogenase (Mdh) enzyme or a methanol oxidase (Mox) enzyme, and wherein the second enzyme is a dihydroxyacetone synthase (Das) enzyme or a transketolase enzyme. The application also describes element and applications, more particularly kits and methods, which include or use the microorganism.
Claims7
A non-naturally occurring microorganism, which has been engineered to acquire methylotrophy, wherein said microorganism comprises a first enzyme and a second enzyme, wherein said first enzyme is a methanol dehydrogenase (Mdh) enzyme, the nucleic acid sequence of which is selected from the group consisting of:
- SEQ ID NOs:
- 34-42 and 45,
- wherein said second enzyme is a dihydroxyacetone synthase (Das) enzyme, the nucleic acid sequence of which is SEQ ID NO:
- 47 or 65,
- wherein at least one of said first and second enzymes is heterologous to said microorganism, and
- wherein the nucleic acid pair of said first enzyme and said second enzyme is selected from the group consisting of SEQ ID NOs:
- 36 and 47, 37 and 47, 40 and 47, 34 and 65, 35 and 65, 36 and 65, 37 and 65, 38 and 65, 39 and 65, 40 and 65, 41 and 65, 42 and 65, and 45 and 65.
Dependencies are inferred from the claim text.