Viral nanoparticle multimers
AssigneeCase Western Reserve University
InventorsNicole F. Steinmetz, Amy M. Wen
Life of this patent
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Abstract
Virus particle multimers and methods of making and using such virus particle multimer are described. Virus particle multimers are constructed by preparing a plurality of asymmetrically functionalized virus particles bearing one or more functional groups and contacting the asymmetrically functionalized virus particles with a first linker molecule that reacts with the functional groups to form a virus particle multimer that includes a plurality of asymmetrically functionalized virus particles connected by the linker molecule. The asymmetrically functionalized virus particles are typically prepared by attaching the virus particles to a support surface to allow asymmetrical functionalization to be introduced.
Claims19
A plant virus particle multimer, the plant virus particle multimer produced by preparing a plurality of asymmetrically functionalized plant virus particles bearing one or more functional groups that are disposed asymmetrically and contacting the asymmetrically functionalized plant virus particles with a first linker molecule that reacts with the functional groups to form a plant virus particle multimer comprising a plurality of asymmetrically functionalized plant virus particles connected by the linker molecule, wherein the one or more asymmetrically disposed functional groups inhibit additional cluster formation and aggregation of the plant virus particles, wherein the plurality of asymmetrically functionalized plant virus particles are prepared by a method comprising the step of attaching a plurality of plant virus particles to a support surface to allow for asymmetrical functionalization of the plant virus particles and releasing the asymmetrically functionalized plant virus particles from the support surface to provide a plurality of unbound asymmetrically functionalized plant virus particles, and wherein the step of preparing a plurality of asymmetrically functionalized plant virus particles is performed at about physiological pH.
A method of delivering a cargo molecule to a cell, comprising contacting the cell with a plant virus particle multimer loaded with the cargo molecule, the plant virus particle multimer produced by preparing a plurality of asymmetrically functionalized virus particles bearing one or more functional groups that are disposed asymmetrically and contacting the asymmetrically functionalized plant virus particles with a first linker molecule that reacts with the functional groups to form a plant virus particle multimer comprising a plurality of asymmetrically functionalized plant virus particles connected by the linker molecule, wherein the one or more asymmetrically disposed functional groups inhibit additional cluster formation and aggregation of the plant virus particles, wherein the plurality of asymmetrically functionalized plant virus particles are prepared by a method comprising the step of attaching a plurality of plant virus particles to a support surface to allow for asymmetrical functionalization of the plant virus particles and releasing the asymmetrically functionalized plant virus particles from the support surface to provide a plurality of unbound asymmetrically functionalized plant virus particles, and wherein the step of preparing a plurality of asymmetrically functionalized plant virus particles is performed at about physiological pH.
Dependencies are inferred from the claim text.