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AssigneeMarshall University Research Corporation
InventorsHongwei D. Yu, Meagan E. Valentine, Richard M. Niles, Thomas Ryan Withers, Brandon D. Kirby
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Bacterial cultures are provided that comprise a modified Pseudomonas aeruginosa bacterium missing or deficient in two or more virulence factors. The two or more virulence factors can be selected from exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, alpha-1,3-rhamnosyltransferase, and 3-phosphoshikimate 1-carboxyvinyltransferase. Certain of the modified Pseudomonas aeruginosa bacteria are also missing or deficient in one or more alginate acetylation enzymes including the alginate O-acetyltransferases AlgI, AlgJ, AlgF, AlgX, and/or the C5-mannuronan epimerase AlgG. Methods of producing alginate are also provided along with compositions comprising alginate produced by the modified Pseudomonas aeruginosa bacteria.
A bacterial culture, comprising a modified Pseudomonas aeruginosa bacterium missing or deficient in a plurality of virulence factors, the plurality of virulence factors comprising exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, and alpha-1,3-rhamnosyltransferase.
A modified Pseudomonas aeruginosa bacterium missing or deficient in a plurality of virulence factors, the plurality of virulence factors comprising exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, and alpha-1,3-rhamnosyltransferase.
A method of producing alginate, comprising culturing a modified Pseudomonas aeruginosa bacterium missing or deficient in a plurality of virulence factors, the plurality of virulence factors comprising exotoxin A, hemolytic phospholipase C, phenazine-specific methyltransferase, and alpha-1,3-rhamnosyltransferase.
Dependencies are inferred from the claim text.