Glycoform purification

A method for the chromatographic purification and/or separation of protein glycoforms by contacting a sample comprising the protein glycoforms with a separation material comprising of a base matrix to the surfaces of which polymer chains are covalently bonded, characterised in that

• the polymer chains comprise end groups —N(Y)—R3 with Y being independently from each other H or CH3, and
• R3 being —CHCOOMR4
• with R4 being C1 to C4 alkyl or C1 to C4 perfluoroalkyl and M being H, Na, K, or NH4.