Device for analyzing single cell using micropores

A device for analyzing a single cell using a micropore, comprising:

• an inlet chamber in which a solution including a target cell is contained;
• an outlet chamber provided on an opposite side of the inlet chamber;
• a pore membrane disposed between the inlet chamber and the outlet chamber and configured to include a micropore thereon;
• a pressure generating means provided in the inlet chamber or the outlet chamber and configured to generate a pressure difference between the inlet chamber and the outlet chamber such that a solution in the inlet chamber flows toward the outlet chamber to pass through the micropore;
• and
• a pair of electrodes respectively disposed in front and rear of the pore membrane and provided to be in electrical contact with the flowing solution to measure an ion conductivity change in front and rear of the pore membrane,
• an inlet channel formed between the inlet chamber and the pore membrane and connected to the inlet chamber to guide flow of the solution containing the target cell;
• and
• an outlet channel formed between the outlet chamber and the pore membrane and connected to the outlet chamber to guide flow of the solution containing the target cell,
• wherein the inlet channel connecting the inlet chamber and the pore membrane to each other is configured in a gradually narrower shape, and
• wherein a diameter D of the micropore is larger than a diameter of the target cell, a thickness t of the pore membrane is 0.5 μm to 1 mm, and a slenderness ratio (t/D) is 0.001 to 5.