Amplification/detection kit, photothermal PCR amplification method and microorganism detection method using the same
AssigneeGwangju Institute of Science and Technology
InventorsMin Gon Kim, Bo Bin Lee
Life of this patent
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Abstract
Disclosed is an amplification/detection kit comprising: a sample pad on which a PCR reaction solution is placed; a transfer pad positioned in contact with the sample pad; a reaction pad into which the PCR reaction solution flowing from the sample pad through the transfer pad flows and which includes a gold thin film deposited on a first surface; and a sealing tape configured to cover and seal a first surface of the transfer pad and a first surface of the reaction pad.
Claims6
A photothermal polymerase chain reaction amplification method using an amplification/detection kit including a gold thin film formed by depositing gold on a pad with an electron-beam, comprising the steps:
- surrounding the gold thin film with a sealing frame to withstand an internal pressure of the pad caused by a high temperature during the photothermal polymerase chain reaction amplification method;
- a first step of irradiating the gold thin film with light having a predetermined intensity and a predetermined wavelength by using a laser configured to radiate the light having the predetermined intensity and the predetermined wavelength for a first period;
- and
- a second step of irradiating the gold thin film with light having the predetermined intensity and the predetermined wavelength by using the laser for a third period after a second period from an end time point of the first period,
- wherein a further step of irradiating the gold thin film with light having the predetermined intensity and the predetermined wavelength by using the laser is not operated for a fourth period from an end time point of the third period,
- wherein the gold thin film has a thickness of 10 nm and is deposited on a glass fiber of the pad, and
- wherein a photothermal polymerase chain amplification reaction occurs in spaces inside the glass fiber.
A microorganism detection method using an amplification/detection kit including a gold thin film formed by depositing gold on a pad with an electron-beam, comprising the steps:
- surrounding the gold thin film with a sealing frame to withstand an internal pressure of the pad caused by a photothermal polymerase chain reaction amplification process;
- a first step of irradiating the gold thin film with light having a predetermined intensity and a predetermined wavelength by using a laser configured to radiate the light having the predetermined intensity and the predetermined wavelength for a first period;
- a second step of irradiating the gold thin film with light having the predetermined intensity and the predetermined wavelength by using the laser for a third period after a second period from an end time point of the first period;
- and
- measuring light emitted from the amplification/detection kit after at least the first and second steps of irradiating are terminated,
- wherein the gold thin film has a thickness of 10 nm and is deposited on a glass fiber of the pad, and
- wherein a photothermal polymerase chain amplification reaction occurs in spaces inside the glass fiber.
Dependencies are inferred from the claim text.