Optimized oncolytic viruses and uses thereof
AssigneeSator Therapeutics
InventorsPeter M. Chumakov, Anastasia V. Lipatova, Stepan P. Chumakov, Natalia D. Tararova, Stephen A. Charles, Anton A. Komar
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Abstract
Methods of inhibiting or reducing tumor growth are disclosed. A composition containing at least one selected oncolytic virus is administered within a tumor of a patient. The virus kills cancerous cells and induces a systemic and lasting anti-tumor immunity that is also compatible with other cancer treatments. Also disclosed are methods of creating synthetic viruses for targeting cancerous tumors.
Claims12
A method of generating a synthetic targeted virus for a cancer cell from a reference virus for a normal cell, comprising:
- for each codon in a given position in the reference virus that codes for a given amino acid, identifying a codon frequency for each codon that codes for the given amino acid in the cancer cell;
- selecting a codon for the given amino acid in the cancer cell that best corresponds to the codon in the reference virus;
- using the selected codon for the given amino acid in the cancer cell in a location in the synthetic targeted virus that matches the given position in the reference virus.
A method of generating an optimized oncolytic virus, comprising:
- (i) culturing a first oncolytic virus on a first cell culture in the presence of a synthetic ribonucleoside or ribonucleotide analog to create mutagenized viruses;
- and
- (ii) culturing the mutagenized viruses on a second cell culture using serial dilution to identify the optimized oncolytic virus.
Dependencies are inferred from the claim text.