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US 12,352,941 B2Granted
Light sheet microscope with line focus
AssigneeMiltenyi Biotec GmbH
InventorsHeinrich Spiecker, Martin Schuette
Life of this patent
Estimating the term…
Abstract
A light sheet microscope for imaging biological materials uses a plurality of light beams, focused to an overlapping line to excite a fluorescent material within the biological sample. The laser-induced fluorescence image is then analyzed and displayed.
Claims17
A light sheet microscope for imaging a biological sample, comprising:
- at least three beams of collimated light propagating along at least three different propagation axes;
- a moveable optical assembly comprising at least three optical subassemblies which focus the at least three beams of light into three line foci forming at least three straight lines, wherein at least one of the three straight lines defines a non-orthogonal angle with respect to its propagation axis and wherein the at least three straight lines are substantially overlapping, and wherein the straight lines and the propagation axes define an excitation plane of the light sheet microscope, and wherein the three substantially overlapping lines are moved laterally in a sequence by moving the movable optical assembly laterally, but wherein the substantially overlapping lines remain in the excitation plane.
A light sheet microscope for imaging a biological sample, comprising:
- at least two collimated light sources each emitting a beam of light along at least two different propagation axes;
- at least two optical subassemblies which focus the at least two beams of light into line foci forming at least two straight lines, wherein at least one of the two straight lines defines a non-orthogonal angle with respect to its propagation axis and wherein the at least two straight lines are substantially overlapping, and wherein the straight lines and the propagation axes define an excitation plane of the light sheet microscope, further comprising a moveable optical assembly which in turn comprises at least one optical sub-assembly, wherein the at least one optical sub-assembly comprises beam shaping elements, wherein the beam shaping elements include at least one anamorphic lens and two spherical lenses, and wherein these beam shaping elements focus at least a third beam of light into at least a line focues that is tilted with respect to the propagation axis.
A light sheet microscope for imaging a biological sample, comprising:
- at least two collimated light sources each emitting a beam of light along at least two different propagation axes;
- at least two optical subassemblies which focus the at least two beams of light into line foci forming at least two straight lines, wherein at least one of the two straight lines defines a non-orthogonal angle with respect to its propagation axis and wherein the at least two straight lines are substantially overlapping, and wherein the straight lines and the propagation axes define an excitation plane of the light sheet microscope, further comprising a movable optical assembly which in turn comprises at least one optical sub-assembly, wherein the at least one optical sub-assembly comprises beam shaping elements, wherein the beam shaping elements include at least one anamorphic lens and two spherical lenses, and wherein these beam shaping elements focus at least a third beam of light into at least a line focus that is tilted with respect to the propagation axis, wherein the three line foci from the three optical subassemblies substantially overlap.
Dependencies are inferred from the claim text.