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US 12,352,758 B2Granted
Quantitative biomarkers of EHV-1 susceptibility and protection
AssigneeCornell University
InventorBettina Wagner
Life of this patent
Estimating the term…
Abstract
The present disclosure is directed to novel biomarkers useful for staging EHV-1 infections and immunity status of horses. This disclosure is further directed to methods of determining EHV-1 infection stage and immunity status of horses using the novel biomarkers.
Claims15
A method comprising:
- (a) providing an intranasal sample from a horse in a herd of horses;
- (b) detecting in said sample equine herpes virus type 1 (EHV-1) specific immunoglobulin G1 (IgG1) and EHV-1 specific immunoglobulin G4/7 (IgG4/7), wherein the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both directed against a glycoprotein C (gC) of EHV-1, and wherein the detection of the EHV-1 specific IgG4/7 and the EHV-1 specific IgG1 is achieved by a multiplex assay based on use of a gC/IL-4 fusion protein;
- (c) determining whether the horse is susceptible or immune to EHV-1 infection, or is undergoing an infection,
- wherein the horse is susceptible to EHV-1 infection or is at an early stage of EHV-1 infection if the levels of the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both below respective threshold levels;
- wherein the horse is at a late stage of EHV-1 infection if the levels of the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both above respective threshold levels;
- and
- wherein the horse is immune to EHV-1 infection, if the level of the EHV-1 specific IgG1 is below a respective threshold level, the level of EHV-1 specific IgG4/7 is above a respective threshold level, and the ratio of the level of the EHV-1 specific IgG4/7 versus the level of the EHV-1 specific IgG1 is more than 10;
- and
- (d) quarantining the horse away from other horses in the herd during an EHV-1 outbreak if the horse is susceptible to EHV-1 infection or at an early or late stage of EHV-1 infection, wherein the horse is quarantined for at least 14 days if the horse is susceptible to EHV-1 infection or is at an early stage infection, and the horse is quarantined for not more than 14 days if the horse is at a late stage of EHV-1 infection;
- and
- not quarantining the horse if the horse is immune to EHV-1 infection.
8 more elements
A method comprising,
- (a) providing an intranasal sample from a horse in a herd of horses;
- (b) detecting in said sample EHV-1 specific IgG1, EHV-1 specific IgG4/7, IFN-α, CCL3, and sCD14, wherein the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both directed against a glycoprotein C (gC) of EHV-1, wherein the detection of the EHV-1 specific IgG4/7 and the EHV-1 specific IgG1 is achieved by a multiplex assay based on use of a gC/IL-4 fusion protein;
- (c) determining that the horse is susceptible to EHV-1 infection, when the levels of the EHV-1 specific IgG1, the EHV-1 specific IgG4/7, IFN-α, CCL3 and sCD14 are all below respective threshold levels;
- determining that the horse is at an early stage of EHV-1 infection, when the level of at least one of IFN-α, CCL3 and sCD14 is above a respective threshold level and the levels of the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both below respective threshold levels;
- determining that the horse is at a late stage of EHV-1 infection, when the levels of the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both above respective threshold levels, and the levels of IFN-α, CCL3 and sCD14 are all below respective threshold levels;
- and
- determining that the horse is immune to EHV-1 infection, when the level of the EHV-1 specific IgG4/7 is above a respective threshold level and the ratio of the level of the EHV-1 specific IgG4/7 versus the level of the EHV-1 specific IgG1 is more than 10, and the levels of EHV-1 specific IgG1, IFN-α, CCL3 and sCD14 are all below respective threshold levels;
- and
- (d) quarantining the horse away from other horses in the herd during an EHV-1 outbreak if the horse is susceptible to EHV-1 infection, at an early stage of EHV-1 infection, or at a late stage of EHV-1 infection, wherein the horse is quarantined for at least 14 days if the horse is susceptible to EHV-1 infection or at an early stage of EHV-1 infection, and the horse is quarantined for not more than 14 days if the horse is at a late stage of EHV-1 infection;
- and
- not quarantining the horse during the EHV-1 outbreak if the horse is immune to EHV-1 infection.
8 more elements
A method comprising,
- (a) providing an intranasal sample from a horse in a herd of horses;
- (b) detecting in said sample EHV-1 specific IgG1, EHV-1 specific IgG4/7, IFN-α, CCL2, and sCD14, wherein the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both directed against a glycoprotein C (gC) of EHV-1, wherein the detection of the EHV-1 specific IgG4/7 and the EHV-1 specific IgG1 is achieved by a multiplex assay based on use of a gC/IL-4 fusion protein;
- (c) determining that the horse is susceptible to EHV-1 infection, when the levels of the EHV-1 specific IgG1, the EHV-1 specific IgG4/7, IFN-α, CCL2 and sCD14 are all below respective threshold levels;
- determining that the horse is at an early stage of EHV-1 infection, when the level of at least one of IFN-α, CCL2 and sCD14 is above a respective threshold level and the levels of the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both below respective threshold levels;
- determining that the horse is at a late stage of EHV-1 infection, when the levels of the EHV-1 specific IgG1 and the EHV-1 specific IgG4/7 are both above respective threshold levels, and the levels of IFN-α, CCL2 and sCD14 are all below respective threshold levels;
- and
- determining that the horse is immune to EHV-1 infection, when the level of the EHV-1 specific IgG4/7 is above a respective threshold level and the ratio of the level of the EHV-1 specific IgG4/7 versus the level of the EHV-1 specific IgG1 is more than 10, and the levels of EHV-1 specific IgG1, IFN-α, CCL2 and sCD14 are all below respective threshold levels;
- and
- (d) quarantining the horse away from other horses in the herd during an EHV-1 outbreak if the horse is susceptible to EHV-1 infection, at an early stage of EHV-1 infection, or at a late stage of EHV-1 infection, wherein the horse is quarantined for at least 14 days if the horse is susceptible to EHV-1 infection or at an early stage of EHV-1 infection, and the horse is quarantined for not more than 14 days if the horse is at a late stage of EHV-1 infection;
- and
- not quarantining a horse during the EHV-1 outbreak if the horse is immune to EHV-1 infection.
8 more elements
Dependencies are inferred from the claim text.