Production method of enzymatic reaction using adenosine instead of ATP

A method of producing S-adenosylmethionine (SAM), the method comprises the following steps:

• (1) adding adenosine triphosphate (ATP) regeneration enzymes, adenosine kinase (AK) enzyme, and S-adenosylmethionine synthases (MAT enzyme), and adenosine in proportion in an enzymatic reaction system to carry out production of SAM, wherein:
• adenosine is used instead of ATP and adenosine monophosphate (AMP);
• and
• the enzymatic reaction system is a 100 L enzymatic reaction system that comprises:
• a solution comprising:
• 1.2 kg of methionine;
• 2.5 kg of adenosine;
• 2.0 kg of sodium hexametaphosphate;
• 0.27 kg of ammonium chloride;
• 1.0 kg of magnesium chloride hexahydrate;
• and
• 0.8 kg of potassium hydrogen phosphate;
• wherein the pH of the solution is adjusted to 7.2;
• ATP regeneration enzymes comprising polyphosphate kinase (PPK) enzyme 200 U/L and adenylate kinase (ADK) enzyme 200 U/L;
• AK enzyme 400 U/L;
• and MAT enzyme 400 U/L;
• wherein the enzymatic reaction system is maintained at a temperature of 35° C. and a pH of 7.2;
• (2) separating the ATP regeneration enzymes, AK enzyme, and MAT enzyme from the enzymatic reaction system to produce a filtrate comprising SAM, ATP, adenosine diphosphate (ADP), and AMP, wherein the ATP regeneration enzymes AK enzyme, and MAT enzyme are separated from the enzymatic reaction system with an ultrafiltration membrane in a filter;
• and
• (3) separating and purifying the filtrate of step (2) to obtain SAM.