Unspecific peroxygenase enzyme variants for selective fatty acid epoxidation or hydroxylation
AssigneeConsejo Superior De Investigaciones Cientificas (Csic)
InventorsJuan Carro Aramburu, Alejandro González Benjumea, Carmen Aranda Oliden, Ana Gutiérrez Suárez, Angel T. Martínez Ferrer
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Abstract
The invention relates to a recombinant Marasmius rotula unspecific peroxygenase (rMroURO) and two mutants thereof, wherein said mutants show enhanced selectivity towards either the epoxidation or the (sub)terminal ω/(ω-1)-hydroxylation of unsaturated fatty acids. The invention also refers to the use of these enzyme variants for the specific epoxidation or hydroxylation of fatty acids such as oleic acid, linoleic acid and/or alpha-linolenic acid.
Claims4
A recombinant peroxygenase enzyme that comprises the amino acid sequence shown in SEQ ID NO:
- 2, wherein said enzyme is encoded by a polynucleotide sequence with at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98% or 99% sequence identity with SEQ ID NO:
- 9.
Dependencies are inferred from the claim text.