Gene-modifying endonucleases
AssigneeAmber Bio Inc.
InventorsBasem Al-Shayeb, Jacob Borrajo, Mohammad Kamyab Javanmardi, Kushagra Sharma
Life of this patent
Estimating the term…
Abstract
The disclosure relates to compositions and methods that modify target nucleic acids, as well as methods of detecting nucleic acids. Various compositions are described herein, including compositions comprising endonucleases, endonuclease systems, and chimeric proteins having the endonuclease and a nucleic-acid modulating domain or a nucleic acid modifying domain.
Claims18
A nuclease system, comprising:
- (a) an endonuclease comprising a nucleic acid encoding an endonuclease comprising an endonuclease comprising a sequence, or a fragment or variant thereof, and having at least 90% identity to SEQ ID NO:
- 3 or having about 1 to about 20 amino acid modifications relative to SEQ ID NO:
- 3;
- and
- (b) an RNA molecule comprising a sequence complementary to one strand of a target nucleic acid molecule,
- wherein the endonuclease further comprises a nuclear localization signal (NLS).
A chimeric protein comprising:
- (a) an endonuclease comprising an endonuclease comprising a sequence, or a fragment or variant thereof, and having at least 90% identity to SEQ ID NO:
- 3 or having about 1 to about 20 amino acid modifications relative to SEQ ID NO:
- 3;
- and
- (b) a nucleic acid-modulating domain or a nucleic acid-modifying domain comprising a sequence comprising a catalytic domain, or a fragment or variant thereof,
- wherein (a) and (b) do not naturally occur together in a same reading frame.
A composition comprising a complex comprising chimeric protein and an RNA molecule, wherein the chimeric protein comprises:
- (a) an endonuclease comprising a sequence, or a fragment or variant thereof, and having at least 90% identity to SEQ ID NO:
- 3 or having about 1 to about 20 amino acid modifications relative to SEQ ID NO:
- 3;
- and
- (b) a nucleic acid-modulating domain or a nucleic acid-modifying domain comprising a sequence comprising a catalytic domain, or a fragment or variant thereof,
- wherein (a) and (b) do not naturally occur together in a same reading frame and
- the RNA molecule comprises a sequence complementary to one strand of a target nucleic acid molecule.
A nuclease system, comprising:
- (a) an endonuclease comprising a nucleic acid encoding an endonuclease comprising an endonuclease comprising a sequence, or a fragment or variant thereof, and having at least 90% identity to SEQ ID NO:
- 3 or having about 1 to about 20 amino acid modifications relative to SEQ ID NO:
- 3;
- and
- (b) an RNA molecule comprising a sequence complementary to one strand of a target nucleic acid molecule,
- wherein the nucleic acid is operably linked to a eukaryotic or viral promoter.
A nuclease system, comprising:
- (a) an endonuclease comprising a nucleic acid encoding an endonuclease comprising a sequence having at least 90% identity to SEQ ID NO:
- 3 and having about 1 to about 20 amino acid modifications relative to SEQ ID NO:
- 3;
- and
- (b) an RNA molecule comprising a sequence complementary to one strand of a target nucleic acid molecule.
A nuclease system, comprising:
- (a) an endonuclease comprising a nucleic acid encoding an endonuclease comprising an endonuclease comprising a sequence, or a fragment or variant thereof, and having at least 90% identity to SEQ ID NO:
- 3 or having about 1 to about 20 amino acid modifications relative to SEQ ID NO:
- 3;
- and
- (b) an RNA molecule comprising a sequence complementary to one strand of a target nucleic acid molecule,
- wherein the target nucleic acid molecule is a eukaryotic nucleic acid molecule.
Dependencies are inferred from the claim text.