Cell body targeted sensors
AssigneeMassachuetts Institute of Technology
InventorsOr A. Shemesh, Changyang Linghu, Kiryl D. Piatkevich, Edward S. Boyden, Won Min Park
Life of this patent
Estimating the term…
Abstract
The invention, in some aspects, relates to polypeptide molecules and their encoding nucleic acid molecules and use of such molecules to direct and localize sensor molecules in the soma of cells in which they are expressed. Compositions of the invention may be delivered to cells and subjects and used in methods to determine activity in cells in which they are expressed.
Claims8
A composition comprising a soma-targeting polypeptide fusion protein, wherein the soma-targeting polypeptide fusion protein comprises an EE-RR polypeptide or functional variant thereof;
- and an Anktail motif polypeptide or functional variant thereof, wherein the EE-RR polypeptide comprises the amino acid sequence of SEQ ID NO:
- 2;
- the functional variant of the EE-RR polypeptide has at least 90% sequence identity to SEQ ID NO:
- 2;
- the Anktail motif polypeptide comprises the amino acid sequence of SEQ ID NO:
- 1;
- and the functional variant of the Anktail motif polypeptide has at least 90% sequence identity to SEQ ID NO:
- 1.
A nucleic acid molecule comprising a sequence encoding a soma-targeting polypeptide fusion protein, wherein the soma-targeting polypeptide fusion protein comprises an EE-RR polypeptide or functional variant thereof;
- and an Anktail motif polypeptide or functional variant thereof, wherein the EE-RR polypeptide comprises the amino acid sequence of SEQ ID NO:
- 2;
- the functional variant of the EE-RR polypeptide has at least 90% sequence identity to SEQ ID NO:
- 2;
- the Anktail motif polypeptide comprises the amino acid sequence of SEQ ID NO:
- 1;
- and the functional variant of the Anktail motif polypeptide has at least 90% sequence identity to SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.