Optically controllable FGFR stimulation using wireless controlled cellular lighting system
AssigneeThe Johns Hopkins University
InventorsGabsang Lee, Hyesoo Kim, InYoung Choi, HoTae Lim, Alex V. Huynh
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Abstract
The present invention relates to the field of stem cells. More specifically, the present invention provides compositions and methods for using optogenetics to sustain the pluripotency of stem cells. In one embodiment, a vector comprises a nucleotide sequencing encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain.
Claims17
A vector comprising a nucleotide sequence encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain, wherein the vector comprises the nucleotide sequence of SEQ ID NO:
- 1.
A fibroblast growth factor 2 (FGF2) free pluripotent stem cell (PSC) culture system comprising a PSC comprising a vector comprising a nucleotide sequence encoding a fusion protein comprising the intracellular domain of fibroblast growth factor 1 receptor (FGFR1) and a photoactivatable domain, wherein the vector comprises the nucleotide sequence of SEQ ID NO:
- 1;
- and a cell culture illumination source capable of illuminating the PSC with a selected wavelength and intensity of light.
Dependencies are inferred from the claim text.