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US 12,350,680 B2Granted
Assay with rapid temperature change
AssigneeEssenlix Corporation
InventorsStephen Y. Chou, Wei Ding, Yufan Zhang, Ji Qi
Life of this patent
Estimating the term…
Abstract
The present invention provides devices, systems, and methods for rapid and easy-to-use in sample thermal cycling or temperature changes for the facilitation of reactions such as but not limited to PCR.
Claims90
A device for changing temperature of a thin fluidic sample, comprising:
- a first plate, a second plate, spacers, and a clamp, wherein:
- (i) the first plate and the second plate are movable relative to each other into different configurations, including an open configuration and a closed configuration;
- (ii) each of the plates comprises, on its respective inner surface, a sample contact area for contacting a fluidic sample;
- (iii) one or both of the plates comprise the spacers on the inner surface thereof;
- and
- (iv) the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns, wherein at least one of the spacers is inside the sample contact area
- wherein in an open configuration, the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers;
- wherein in a closed configuration, the two plates are operable to compress at least a part of the sample into a layer of substantially uniform thickness that is substantially stagnant relative to the plates, wherein the thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
- wherein the inner surface of the first plate faces the inner surface of the second plate in the closed configuration;
- wherein the clamp compresses the first plate and the second plate to fix the two plates together in the closed configuration, wherein the pressure of the clamp applied on the plates is 0.01 kg/cm2 or higher;
- wherein at least one of the first and second plates comprise a radiation absorbing layer that is configured to absorb electromagnetic wave from a radiation source and convert a substantial portion of the electromagnetic wave into heat and have an average ascending temperature ramp rate of at least 10° C./s;
- and
- wherein the radiation absorbing layer comprises a disk-coupled dots-on-pillar antenna (D2PA) array.
10 more elements
A device for changing temperature of a thin layer of a fluidic sample, comprising:
- a first plate, a second plate, and spacers, wherein:
- (i) the first plate has a thickness of 100 um (micron) or less;
- (ii) the first plate and the second plate are movable relative to each other into different configurations, including an open configuration and a closed configuration;
- (iii) each of the plates comprises, on its respective inner surface, a sample contact area for contacting a fluidic sample;
- (iv) one or both of the plates comprise the spacers that are fixed to the respective inner surface thereof;
- (v) the spacers have a predetermined substantially uniform height that is equal to or less than 200 microns;
- (vi) at least one of the spacers is inside the sample contact area;
- wherein in an open configuration, the two plates are partially or completely separated apart, the spacing between the plates is not regulated by the spacers;
- and
- wherein in a closed configuration, which is configured after the sample is deposited in the open configuration, at least a part of the sample is compressed by the two plates into a layer of substantially uniform thickness and is substantially stagnant relative to the plates, wherein the thickness of the layer is confined by the sample contact areas of the two plates and is regulated by the plates and the spacers;
- wherein at least one of the first and second plates comprise a radiation absorbing layer that is configured to absorb electromagnetic wave from a radiation source and convert a substantial portion of the electromagnetic wave into heat and have an average ascending temperature ramp rate of at least 10° C./s;
- and
- wherein the radiation absorbing layer comprises a disk-coupled dots-on-pillar antenna (D2PA) array.
10 more elements
A device for changing temperature of a thin layer of a fluidic sample, comprising:
- a first plate, a second plate, and a clamp, wherein:
- (i) the first plate and the second plate are movable relative to each other into different configurations, including an open configuration and a closed configuration;
- (ii) each of the plates comprises, on its respective surface, a sample contact area for contacting a fluidic sample to be assayed;
- and
- (iii) the clamp compresses the first plate and the second plate to fix the plates at the closed configuration, wherein the pressure of the clamp applied on the plates is 0.01 kg/cm2 or higher,
- wherein at least one of the first and second plates comprise a radiation absorbing layer that that is configured to absorb electromagnetic wave from a radiation source and convert a substantial portion of the electromagnetic wave into heat and have an average ascending temperature ramp rate of at least 10° C./s;
- wherein the radiation absorbing layer comprises a disk-coupled dots-on-pillar antenna (D2PA) array;
- wherein in an open configuration, the two plates are partially or completely separated apart, the average spacing between the plates is 250 μm or larger;
- and
- wherein in a closed configuration, which is configured after the sample is deposited in the open configuration, at least a part of the sample is compressed by the two plates into a layer of substantially uniform thickness and is substantially stagnant relative to the plates, wherein the thickness of the layer is confined by the sample contact areas of the two plates and is 200 μm thick or less.
7 more elements
Dependencies are inferred from the claim text.