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US 12,350,672 B2Granted
Systems and methods related to continuous flow droplet reaction
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsCody Youngbull, Andrew Hatch, Andrew Larsen, Tathagata Ray, Matthew Underhill
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Abstract
Described herein are systems relating to a continuous-flow instrument that includes all necessary components for digital droplet quantification without the need to introduce key reagents or collect and transfer droplets between stages of instrument operation. Digital quantification can proceed without any additional fluid or consumable handling and without exposing fluids to risk of external contamination.
Claims20
A method for conducting an assay on a biological sample, comprising:
- (a) providing a first injector comprising a first dispersed phase and a first continuous phase, and a second injector comprising a second dispersed phase and a second continuous phase, wherein said first dispersed phase comprises said biological sample and said second dispersed phase comprises one or more reagents for a reaction;
- (b) directing (i) the first dispersed phase from said first injector to a coalescer, and (ii) the second dispersed phase from said second injector to said coalescer, wherein said coalescer coalesces said first dispersed phase and said second dispersed phase to yield a fluid comprising said biological sample;
- (c) directing said fluid from said coalescer to a reactor downstream of said coalescer;
- (d) in said reactor, subjecting said fluid to conditions sufficient to conduct said reaction on the biological sample;
- and
- (e) using a detector that is in fluid communication with the reactor to detect said biological sample or a derivative thereof.
3 more elements
Dependencies are inferred from the claim text.