Cartridges for cell processing

A cartridge for use in cell processing, comprising:

• a plurality of cell processing modules defining an enclosed housing, wherein at least one module of the plurality of cell processing modules is selected from the group consisting of a cell selection module, an electroporation module, and a counterflow centrifugal elutriation (CCE) module, and at least one module of the plurality of cell processing modules comprises a bioreactor module;
• a liquid transfer bus comprising a manifold, at least one fluid conduit, and at least one valve;
• and
• a pump system comprising an opening in the enclosed housing and tubing in fluidic communication with the liquid transfer bus, wherein at least a portion of the tubing is externally exposed from the opening of the housing so as to be engageable by a pump actuator of an instrument within a cell processing workcell to move fluid within the cartridge,
• wherein each cell processing module is fluidically coupled to the manifold of the liquid transfer bus, and
• wherein the cartridge is sized and shaped to pass through a feedthrough of the cell processing workcell.