Gene sequencing chip and gene sequencing method

A gene sequencing chip, comprising:

• an upper substrate including a plurality of liquid inlets for inletting liquid drops,
• a lower substrate opposite to the upper substrate and spaced therefrom by a gap for accommodating the liquid drops, the liquid drops comprising raw material liquid drops, gene library preparation reagent liquid drops, gene amplification reagent liquid drops and gene sequencing reagent liquid drops,
• wherein the lower substrate comprises a liquid drop operation region, the liquid drop operation region comprises:
• at least one first manipulation electrode array, each first manipulation electrode array comprising a plurality of first manipulation electrodes arranged in a row in a first direction and spaced apart from each other, the first manipulation electrode array being configured to move the raw material liquid drops and the gene library preparation reagent liquid drops;
• at least one second manipulation electrode array, each second manipulation electrode array comprising a plurality of second manipulation electrodes arranged in a row in the first direction and spaced apart from each other, the second manipulation electrode array being configured to move the gene amplification reagent liquid drops and the gene sequencing reagent liquid drops;
• and
• a rectangular ring-shaped array comprising a plurality of third manipulation electrodes spaced apart from each other and forming a rectangular shape, the rectangular ring-shaped array being configured to mix the raw material liquid drops and the gene library preparation reagent liquid drops,
• wherein the second manipulation electrode array and the first manipulation electrode array are adjacent to each other in a second direction perpendicular to the first direction,
• wherein the lower substrate further comprises an electrode control region for controlling an energizing sequence for the plurality of first manipulation electrodes, the plurality of second manipulation electrodes and the plurality of third manipulation electrodes to achieve movement of the liquid drops.