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US 12,350,342 B2Granted
O-linked glycosylation recognition motifs
AssigneeVaxnewmo LLC
InventorChristian Harding
Life of this patent
Estimating the term…
Abstract
Provided herein are glycoproteins containing O-linked glycosylation recognition motifs, and methods of making, for example, for use in the production of conjugate vaccines.
Claims35
A bioconjugate comprising an oligo- or polysaccharide covalently linked to a fusion protein:
- wherein the fusion protein comprises a ComP protein (ComP) glycosylation tag comprising an isolated fragment or variant thereof of a ComP protein;
- wherein the ComP glycosylation tag has a length of between 18 and 50 amino acids;
- wherein the ComP glycosylation tag comprises both a cysteine residue corresponding to the conserved cysteine residue at position 71 of SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1) and a cysteine residue corresponding to the conserved cysteine residue at position 93 of SEQ ID NO:
- 2;
- wherein the fusion protein is glycosylated with the oligo- or polysaccharide on the ComP glycosylation tag at a serine residue corresponding to the conserved serine residue at position 82 of SEQ ID NO:
- 2;
- wherein the ComP glycosylation tag does not comprise a methionine residue corresponding to the conserved methionine residue at position 104 of SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1);
- and
- wherein the fusion protein of the bioconjugate does not comprise, in relationship to the ComP glycosylation tag, a methionine residue at a position that would correspond to or correspond about to the conserved methionine residue at position 104 of SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1).
12 more elements
A fusion protein comprising a ComP glycosylation tag comprising an isolated fragment or variant thereof of a ComP protein,
- wherein the fragment comprises a serine residue corresponding to the conserved serine residue at position 82 in SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1) and both a cysteine residue corresponding to the conserved cysteine residue at position 71 of SEQ ID NO:
- 2 and a cysteine residue corresponding to the conserved cysteine residue at position 93 of SEQ ID NO:
- 2;
- wherein the ComP glycosylation tag has a length of between 18 and 50 amino acids;
- wherein the ComP glycosylation tag does not comprise a methionine residue corresponding to the conserved methionine residue at position 104 of SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1);
- and
- wherein the fusion protein does not comprise, in relationship to the ComP glycosylation tag, a methionine residue at a position that would correspond to or correspond about to the conserved methionine residue at position 104 of SEQ ID NO:
- 2 (ComP110264:
- ENV58402.1).
10 more elements
Dependencies are inferred from the claim text.