Recombinant high growth rate influenza virus comprising mutations in M1, NS2, and PB2
AssigneesVivaldi Biosciences Inc., Bluesky Immunotherapies GmbH
InventorsThomas Muster, Amy Aspelund, Markus Wolschek
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Abstract
The present invention provides high growth influenza reassortant virus and high growth influenza reassortant virus vectors comprising amino acid modifications in the PB2, PB1, M1 and/or NS2 proteins which exhibit highly increased growth rates compared to unmodified influenza virus. Further provided are pharmaceutical compositions comprising reassortant virus and viral vectors comprising said modifications and their use for vaccination purposes.
Claims14
A recombinant influenza B virus with increased growth rate in Vero cells as compared to wild type influenza virus comprising SEQ ID NOs:
- 2, 6 and 10, and lacking a functional NS1 protein (delNS1 influenza), comprising:
- an M gene segment resulting in an M1 protein comprising amino acid sequence SEQ ID NO:8;
- and
- an NS gene segment comprising one or more nucleotide modifications resulting in an NS2 protein comprising one of amino acid sequences SEQ ID NO:12 or 34, and optionally
- a PB2 gene segment resulting in a PB2 protein comprising amino acid sequence SEQ ID NO:4.
Dependencies are inferred from the claim text.