Animal model for hepatocellular carcinoma and uses thereof
AssigneesTaipei Veterans General Hospital, National Yang-Ming University
InventorsJaw-Ching Wu, Yu-Wei Chiou
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Abstract
The invention provides an animal model for hepatocellular carcinoma and uses thereof. The genome of the animal model includes a hepatitis B virus genome and a single set of endogenous miR-122 that is partially deleted. Due to the early and high incidence of spontaneous hepatocellular carcinoma in the animal model and its abnormal fat metabolism, the animal model can be used to screen candidate agents for prevention or treatment of hepatitis B virus and hepatocellular carcinoma or other diseases or to develop uses for diagnosis and prediction of hepatocellular carcinoma.
Claims10
A method for producing a transgenic animal model of mouse or rat for hepatocellular carcinoma, comprising:
- a) providing a polynucleotide expression vector encoding a hepatitis B virus (HBV) genome comprising enhancer I/II, open reading frames X, C, PS, and S at the 5′ end and a polyadenylation site at the 3′ end;
- b) introducing the polynucleotide expression vector into a genetic locus of a genome of an animal to produce a transgenic animal containing a genome encoding the HBV genome;
- c) providing an endogenous miR-122 knockout animal, which is the same species as the transgenic animal of step b);
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- d) hybridizing the transgenic animal containing the genome encoding the HBV genome and the endogenous miR-122 knockout animal to produce a transgenic animal having a genome encoding the HBV genome and the endogenous miR-122 knockout.
Dependencies are inferred from the claim text.