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US 12,347,665 B2Granted
Rapid identification and sequence analysis of intact proteins in complex mixtures
AssigneesUniversity of Virginia Patent Foundation, Thermo Finnigan LLC
InventorsDonald F. Hunt, Ann M. English, Scott A. Ugrin, Dina L. Bai, Jeffrey Shabanowitz, John Edward Philip Syka
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Abstract
The present disclosure relates to novel and improved methods of analyzing proteins, peptides and polypeptides by mass spectrometry using ion-ion reactions. More specifically the disclosure relates to improved methods for implementing the m/z selective arresting of ion-ion reactions within the ion-ion reaction cell of a mass spectrometer system during a period where ion-ion reactions are performed.
Claims11
A method for mass spectrometry analysis, comprising:
- introducing ions into a reaction cell, the ions including analyte precursor ions and reagent ions;
- applying radio-frequency (RF) voltages to electrodes of the reaction cell to generate an RF field, such that ions present within the reaction cell have oscillatory motion frequencies that are primarily determined by their mass-to-charge ratios;
- and
- while applying the RF voltages:
- generating a first auxiliary AC dipole field, oriented in a first dimension, having at least one component frequency selected to kinetically excite analyte product ions formed by reaction of the analyte precursor ions with the reagent ions;
- generating a second auxiliary AC dipole field oriented in a second dimension orthogonal to the first dimension, the second auxiliary dipole field having at least one component frequency selected to kinetically excite reagent ions, and wherein the component frequencies of the first and second auxiliary AC dipole fields differ from one another.
3 more elements
Dependencies are inferred from the claim text.