US 12,345,671 B2 · Analyte sensors for detecting asparagine and aspartate and methods of use thereof

Abstract

The present disclosure provides an analyte sensor for use in detecting aspartate and/or asparagine. In certain embodiments, an aspartate-responsive active site of a presently disclosed analyte sensor includes an aspartate oxidase disposed upon a surface of a working electrode. In certain embodiments, an asparagine-responsive active site of a presently disclosed analyte sensor includes an enzyme system comprising an aspartate oxidase and an asparaginase disposed upon a surface of a working electrode. The present disclosure further provides methods for detecting aspartate and/or asparagine using the disclosed analyte sensors.

Claims13

Dependent claims

An analyte sensor for detecting asparagine in vivo, the sensor comprising:

(i) at least a first working electrode;
(ii) at least one asparagine-responsive active area for detecting asparagine, the asparagine-responsive active area disposed upon a surface of the first working electrode, wherein the asparagine-responsive active area comprises aspartate oxidase and asparaginase;
and
(iii) a mass transport limiting membrane permeable to asparagine that overcoats at least the asparagine-responsive active area,
wherein the sensor is configured to be partially inserted into a user's skin such that a distal portion of the sensor is in contact with interstitial fluid to detect asparagine in vivo.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/561,505Filed 23 Dec 2021 · us provisional application

Priority

30 Dec 2020

Published

US 2022/0205944 A130 Jun 2022

Granted

1 Jul 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12Q 1/004
Enzyme electrodes: mediator-assisted
G01N 27/3273
Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood: devices therefor, e.g. test element readers, circuitry
A61B 5/1486
Using chemical or electrochemical methods, e.g. by polarographic means: using enzyme electrodes, e.g. with immobilised oxidase
C12N 9/0022
Acting on the CH-NH2 group of donors: with oxygen as acceptor

Analyte sensors for detecting asparagine and aspartate and methods of use thereof

Life of this patent

Analyte sensors for detecting asparagine and aspartate and methods of use thereof

Life of this patent

Abstract

Claims13