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US 12,345,641 B2Granted
Fluorescence quenching immunoassay
AssigneeIDEZZ Laboratories, Inc.
InventorsJennifer E. Gagner, Kiamars Hajizadeh, Evan M. Peck, James R. Salter, Paul J. Travers, Christopher P. Turmel, Yingzi Wu, Hongzhi Xie, Murthy V. S. N. Yerramilli
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Abstract
The present invention relates to a method and reagents for determining the presence of or the amount of an analyte in a sample.
Claims19
A method for determining the presence of or the amount of an analyte in a sample comprising:
- (i) providing a sample suspected of containing an analyte;
- (ii) contacting the sample with a fluorescent tracer and a binding partner to provide an assay composition;
- wherein the binding partner is specific for the analyte and the fluorescent tracer;
- (iii) irradiating the assay composition with light at a first wavelength;
- and
- (iv) measuring the intensity of light emitted at a second wavelength,
- wherein
- (a) the intensity of the light emitted at the second wavelength is greater if the fluorescent tracer is not bound to the binding partner than if the fluorescent tracer is bound to the binding partner,
- (b) the light at the first wavelength is not linearly polarized, and
- (c) the fluorescent tracer is selected from the group consisting of:
- wherein T is a bond or a linking group and E is an epitopic moiety capable of specifically binding to the binding partner;
- and
- wherein the intensity of the light emitted at the second wavelength is directly proportional to the concentration of the analyte in the sample.
10 more elements
A method for determining the presence of or the amount of an analyte in a sample comprising:
- (i) providing a sample suspected of containing an analyte;
- (ii) contacting the sample with a fluorescent tracer and a binding partner to provide an assay composition;
- wherein the binding partner is specific for the analyte and the fluorescent tracer;
- (iii) irradiating the assay composition with light at a first wavelength;
- and
- (iv) measuring the intensity of light emitted at a second wavelength,
- wherein
- (a) the intensity of the light emitted at the second wavelength is greater if the fluorescent tracer is not bound to the binding partner than if the fluorescent tracer is bound to the binding partner,
- (b) the light at the first wavelength is not linearly polarized,
- (c) the fluorescent tracer is selected from the group consisting of:
- wherein T is a bond or a linking group and E is an epitopic moiety capable of specifically binding to the binding partner;
- (d) the intensity of the light emitted at the second wavelength is directly proportional to the concentration of the analyte in the sample;
- and
- (e) wherein the analyte is selected from the group consisting of SDMA, melamine, T4, bile acid, biotin, thyroxine, cystatin-B, NT-proBNP, antibiotics, sulfadimethoxine, cortisol, and progesterone.
11 more elements
Dependencies are inferred from the claim text.