Genetic engineering bacterium for de novo synthesis of cis,cis-muconic acid by taking glucose as substrate and applications thereof

A genetically engineered bacterium utilizing glucose for de novo synthesis of cis, cis-muconic acid, wherein

• the genetically engineered bacterium is Corynebacterium glutamicum ATCC13032 recombinantly modified to comprise a cis,cis-muconic acid pathway construction module and an intermediate high-yield module and further having knockouts of pyk, aroE, pcaG/H and CatB genes;
• wherein
• the cis,cis-muconic acid pathway construction module comprises a protocatechuate decarboxylase gene comprising SEQ ID NO:
• 37, an UbiX-like flavin prenyltransferase gene comprising SEQ ID NO:
• 68 or SEQ ID NO:
• 38, a 4-hydroxybenzoate decarboxylase subunit D gene comprising SEQ ID NO:
• 69 or SEQ ID NO:
• 39, and a catechol 1,2-dioxygenase gene comprising SEQ ID NO:
• 76 or SEQ ID NO:
• 35;
• and
• the intermediate high-yield module comprises a 3-dehydroquinate synthase gene comprising SEQ ID NO:
• 51, a 3-dehydroquinate dehydratase gene comprising SEQ ID NO:
• 52, and a transketolase gene comprising SEQ ID NO:
• 53.