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US 12,344,871 B2Granted
Method for producing cysteine from glutathione
AssigneesAmano Enzyme Inc., Amano Enzyme Europe Ltd.
InventorHiroki Fujioka
Life of this patent
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Abstract
It is an object of the present invention to provide a practical means of producing cysteine from glutathione that is also suitable for use in the field of foods. Cysteine is produced from glutathione by a first step of producing cysteinylglycine by the action of a γ-glutamyl peptidase derived from a microorganism on reduced glutathione; and a second step of producing cysteine by the action of an acid protease derived from a microorganism on the cysteinylglycine.
Claims2
A method for producing cysteine from glutathione comprising:
- adding a glutaminase derived from Bacillus amyloliquefaciens and an acid protease derived from Aspergillus oryzae to a glutathione-containing composition or a solution thereof, or a glutathione solution;
- a first step of producing cysteinylglycine by reacting the glutaminase derived from Bacillus amyloliquefaciens on the glutathione-containing composition or a solution thereof, or a glutathione solution to produce cysteinylglycine;
- and
- a second step of producing cysteine by reacting the acid protease derived from Aspergillus oryzae on the cysteinylglycine,
- wherein the first step and the second step are simultaneously performed, and wherein the first and second reactions are carried out at a pH of 4 to 6.
2 more elements
Dependencies are inferred from the claim text.