Compositions comprising a CRISPR nuclease and uses thereof
AssigneeArbor Biotechnologies, Inc.
InventorsShaorong Chong, Quinton Norman Wessells, Roy Ziblat, Noah Michael Jakimo, Anthony James Garrity, Lauren E. Alfonse
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Abstract
The present disclosure relates to variant polypeptides, methods of preparing the variant polypeptides, processes for characterizing the variant polypeptides, compositions and cells comprising the variant polypeptides, and methods of using the variant polypeptides. The disclosure further relates to complexes comprising the variant polypeptides, methods of producing the complexes, processes for characterizing the complexes, cells comprising the complexes, and methods of using the complexes.
Claims20
A variant polypeptide that comprises a substitution that corresponds to a substitution in the polypeptide of SEQ ID NO:
- 3 selected from D509R, S511R, I521R, D535G, Q514G, L516R, L516G, E198R, S527R, D509K, D509G, L580G, V359R, D535K, Y381R, R354G, M380K, M380R, V383R, L580R, E367R, 1521K, E367G, E507R, I521G, W350R, D535R, R528K, S527K, L385G, W350G, L516K, Y381G, H532R, D129R, P615R, G578R, M380G, V595G, R531G, D129G, R531K, D158G, N476G, G578K, A512R, P618R, V595R, V595K, V383G, A597G, Y381K, P618G, E198K, T288R, E507K, L385R, C538G, P615G, D386R, S511K, C371G, K136G, N220R, S78K, K141G, K240R, D277R, T165R, and K374R, and wherein the variant polypeptide comprises:
- (a) at least 98% sequence identity to the polypeptide of SEQ ID NO:
- 3;
- or
- (b) an amino acid sequence that differs from SEQ ID NO:
- 3 solely by the modification of 1-13 amino acids within SEQ ID NO:
- 3;
- and
- wherein the variant polypeptide has nuclease activity.
Dependencies are inferred from the claim text.