Artificially synthesized mRNA and use of same
AssigneePublic University Corporation Nagoya City University
InventorsShinichi Hoshino, Nao Hosoda
Life of this patent
Estimating the term…
Abstract
Provided is a technique for improving the translation efficiency of mRNA. The mRNA includes: a 5′ untranslated region of an mRNA encoding a protein; and a 3′ untranslated region having 40% or more and 80% or less complementarity to 5′ untranslated region.
Claims11
An artificially synthesized mRNA comprising a 5′ untranslated region (5′ UTR) of an mRNA encoding a protein, wherein the 5′ UTR of comprises SEQ ID NO:
- 27 and encoding glyceraldehyde-3-phosphate dehydrogenase;
- and
- a 3′ untranslated region (3′ UTR) having 50% or more and 80% or less complementarity to the 5′ UTR.
A method for manufacturing an artificially synthesized mRNA, comprising the step of:
- preparing an artificially synthesized mRNA which comprises:
- a 5′ untranslated region (5′ UTR) of an mRNA encoding a protein;
- and a 3′ untranslated region (3′ UTR) having 50% or more and 80% or less complementarity to the 5′ UTR, wherein the 5′ UTR comprises SEQ ID NO:
- 27 and encoding glyceraldehyde-3-phosphate dehydrogenase.
A method for manufacturing an artificially synthesized mRNA, comprising the step of:
- preparing an mRNA which comprises a 5′ untranslated region (5′ UTR) of an mRNA, wherein the 5′ UTR comprises SEQ ID NO:27 and encoding glyceraldehyde-3-phosphate dehydrogenase;
- and a 3′ untranslated region (3′ UTR) of the mRNA and encoding glyceraldehyde-3-phosphate dehydrogenase.
A method comprising the step of:
- introducing an artificially synthesized mRNA comprising a 5′ untranslated region (5′ UTR) of an mRNA encoding glyceraldehyde-3-phosphate dehydrogenase, wherein the 5′ UTR comprises SEQ ID NO:27;
- and a 3′ untranslated region (3′ UTR) of the mRNA encoding glyceraldehyde-3-phosphate dehydrogenase into an isolated cell, wherein said mRNA further comprises a coding region containing a gene of interest located between said 5′ UTR and said 3′ UTR and said gene of interest can be expressed in said isolated cell.
An isolated cell comprising an artificially synthesized mRNA comprising a 5′ untranslated region (5′ UTR) of an mRNA encoding glyceraldehyde-3-phosphate dehydrogenase, wherein the 5′ UTR comprises SEQ ID NO:27 and a 3′ untranslated region (3′ UTR) of the mRNA encoding glyceraldehyde-3-phosphate dehydrogenase introduced thereinto, wherein said mRNA further comprises a coding region containing a gene of interest located between said 5′ UTR and said 3′ UTR and said gene of interest can be expressed in said isolated cell.
Dependencies are inferred from the claim text.