US 12,344,862 B2 · Human intestinal epithelium model and method for preparing same

Abstract

The present invention relates to a method for preparing a human intestinal epithelial model. The human intestinal epithelial model, prepared by the method according to the present invention, has all characteristics of goblet cells, enteroendocrine cells, and Paneth cells, and thus can highly mimic the function of actual human intestinal cells, so that the human intestinal epithelial model can be effectively used for development of new drugs, evaluation of drug absorption and toxicity, or evaluation of engraftment of intestinal microorganisms, or as a composition for in vivo transplantation.

Claims5

Dependent claims

A method for differentiating human intestinal epithelial cell (hIEC) progenitors from definitive endoderm cells, comprising:

culturing the definitive endoderm cells in a medium comprising epidermal growth factor (EGF), R-spondin 1, and insulin,
wherein the medium does not comprise any additive selected from the group consisting of Wnt-3A, Noggin, and SB202190, and
wherein the definitive endoderm cells are cultured for a sufficient amount of time to establish hIEC progenitors.

Dependencies are inferred from the claim text.

Facts

Application

17/087,893Filed 3 Nov 2020

Published

US 2022/0135949 A15 May 2022

Granted

1 Jul 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification18 codes

C12N 5/0679
Vertebrate cells: cells of the gastro-intestinal tract
C12N 5/068
Cells of the gastro-intestinal tract: stem cells; Progenitors
A61K 35/38
Digestive system: stomach; Intestine; Goblet cells; Oral mucosa; Saliva
A61K 35/545
Ovaries; Ova; Ovules; Embryos; Foetal cells; Germ cells: embryonic stem cells; Pluripotent stem cells; Induced pluripotent stem cells;…

Human intestinal epithelium model and method for preparing same

Life of this patent

Human intestinal epithelium model and method for preparing same

Life of this patent

Abstract

Claims5