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US 12,344,852 B2Granted
Control of phenotype in plants
AssigneeGreenvenus, LLC
InventorsSekhar Boddupalli, Andrey Boukharov, Rio Stamler, Zhongsen Li, Arianne Tremblay, Stephen Schauer, Shiv B. Tiwari, John Salmeron
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Abstract
The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest “on-demand” by treatment of the plant with a chemical ligand.
Claims16
One or more vectors or polynucleotides comprising:
- (a) a polynucleotide encoding a VGEvy gene switch, comprising:
- (i) a transactivation domain;
- (ii) a DNA-binding domain;
- and
- (iii) a ligand-binding domain having the amino acid sequence of SEQ ID NO:2;
- (b) a nucleic acid encoding a protein or an RNA that inhibits a fungus;
- and
- (c) a regulatory element operably connected 5′ of (b)
- wherein the VGEvy gene switch induces expression of (b) in the presence of a chemical ligand.
6 more elements
Dependencies are inferred from the claim text.