Cell-associated heterologous food and/or feed enzymes
AssigneeDanstar Ferment AG
InventorsAaron Argyros, Michelle Oeser, Erin Wiswall, Janet Fisher, Johannes Van Eijk, J. Kevin Kraus, Kevin Wenger, Brooks Henningsen, Ryan Skinner
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Abstract
The present disclosure concerns recombinant yeast host cells expressing cell-associated heterologous food and/or feed enzymes which are expressed during the propagation phase of the recombinant yeast hosts cells. The recombinant yeast host cells can be used in a subsequent production process to make food and/or feed products, for example, baked products.
Claims15
A process for making a food or a feed product, said process comprising including a recombinant yeast host cell or an additive comprising the recombinant yeast host cell in the food or the feed product, wherein the recombinant yeast host cell has a heterologous nucleic acid molecule encoding a cell-associated heterologous maltogenic alpha-amylase, wherein the maltogenic alpha-amylase has at least 80% identity to the amino acid sequence of SEQ ID NO:
- 1, 51, 65 or 108, wherein the heterologous nucleic acid molecule is operatively associated with a promoter allowing expression of the heterologous nucleic acid molecule during propagation under aerobic conditions.
Dependencies are inferred from the claim text.