Highly knotted molecular topologies from single-stranded nucleic acids
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsHao Yan, Fei Zhang, Xiaodong Qi
Life of this patent
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Abstract
In some embodiments, complex molecular knots with high crossing numbers are achieved by folding, following a prescribed folding order, single-stranded DNA or RNA of customized sequences into target shapes. Such complex molecular knots with high crossing numbers are useful for biomedical applications including use as immunostimulatory agents and/or protein hosts and carriers.
Claims17
A knotted, self-assembled single-stranded nucleic acid (ssNA) nanostructure comprising a crossing number from 9 to 67 and comprising at least one paranemic cohesion crossover,
- wherein the ssNA is selected from DNA or RNA,
- wherein the ssNA length is from about 1500 to about 8000 nucleotides,
- wherein the nanostructure self-assembles by means of paranemic cohesion into knots comprising four ssNA regions, and
- wherein the ssNA comprises a sequence at least about 99% identical to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:
- 10, SEQ ID NO:11, or SEQ ID NO:16.
A complex comprising a knotted, self-assembled single-stranded nucleic acid (ssNA) nanostructure comprising a crossing number from 9 to 67 and comprising at least one paranemic cohesion crossover,
- wherein the ssNA is selected from DNA or RNA,
- wherein the ssNA length is from about 1500 to about 8000 nucleotides,
- wherein the nanostructure self-assembles by means of paranemic cohesion into knots comprising four ssNA regions, and a topological control strand comprising a sequence at least 99% identical to SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14 or SEQ ID NO:15.
Dependencies are inferred from the claim text.