Cell culture device, system and methods of use thereof

A method of treating a medical, aesthetic or veterinary condition in a subject comprising administering cells to the subject from a container comprising the cells via a cannula in which the container comprises a base section, a top section arranged in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, in which the bottom section of the container is provided with the cannula, in which the wall element comprises a plurality of lateral rigid sections in the wall element arranged in parallel with the base section where each pair of lateral rigid sections is interleaved with a deformable region, and in which the wall element is a gas permeable material.

A method of obtaining a biological sample from a subject comprising inserting a cannula into the subject in which the cannula is disposed within a container having a base section, a top section arranged in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, in which the bottom section of the container is provided with the cannula, in which the wall element comprises a plurality of lateral rigid sections in the wall element arranged in parallel with the base section, where each pair of lateral rigid sections is interleaved with a deformable region, in which the wall element is a gas permeable material, and in which said container is operatively connected to an actuator means for expanding the container thereby removing the sample from the subject.

A cell culture container having a base section, a top section arranged in parallel with the base section and a wall element arranged between the top section and the base section and defining an internal lumen of the container, in which the wall element of the container is compressible with respect to the top and bottom section, and in which the top section of the container has an optionally sealable inlet, in which the wall element comprises a plurality of lateral rigid sections in the wall element arranged in parallel with the base section, where each pair of lateral rigid sections is interleaved with a deformable region, and in which the wall element is a gas permeable material.