Functionalized UBX protein materials for enhanced purification of antibodies
AssigneeBondwell Technologies LP
InventorsSarah E. Bondos, David W. Howell
Life of this patent
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Abstract
Provided herein are methods and compositions for purifying antibodies. Purification is achieved by increasing the binding capacity of protein A chromatography by covalent attachment of a protein A domain (E, D, A, B, C), or domain Z, or a functional variant thereof, to Drosophila melanogaster transcription factor Ultrabithorax (Ubx) materials. The compositions include fusion proteins containing Drosophila melanogaster transcription factor Ultrabithorax (Ubx) or a fragment thereof and an immunoglobulin binding protein. In some embodiments, the immunoglobulin binding protein is a protein A domain, a protein Z domain or a fragment thereof.
Claims9
A Ubx biomaterial comprising two or more self-assembled fusion proteins, wherein each fusion protein comprises at least one Ubx protein and an immunoglobulin binding protein, wherein the immunoglobulin binding protein comprises one or more monomeric domains of domain Z or an immunoglobulin binding functional variant thereof, wherein domain Z comprises an amino acid sequence having at least 95% identity to SEQ ID NO:
- 1, and wherein the immunoglobulin binding protein is linked to the N-terminus of the Ubx protein via a linker.
Dependencies are inferred from the claim text.