US 12,343,702 B2 · Anionic exchange-hydrophobic mixed mode chromatography resin

Abstract

Chromatography resins having anionic exchange-hydrophobic mixed mode ligands, that are useful for purifying target biomolecules using anionic exchange (i.e., where the ligand is positively charged) and hydrophobic mixed mode chromatography. The chromatography resins allow for efficient purification of target biomolecules (e.g., recombinant proteins, antibodies, antibody-drug conjugates, or antibody derivatives including, but not limited to, antibody fragments and antibody fusions) from a sample, and have been found to be useful in purifying monomeric target biomolecules from aggregate target biomolecules. In an embodiment, the chromatography resins are useful for separating antibodies from one or more components (e.g., contaminants) in the sample.

Claims14

Dependent claims

A chromatography resin having the following formula:

Chromatography matrix-(X)—N(R1)(R2)—(R3-L)n-Ar
or an anionic salt thereof,
wherein:
X is a spacer from the group consisting of —O—CH2—, —O—CH2—CH2—, —O—CH2—CH2—CH2—, —O—CH2—CH2—CH2—CH2—, —O—CH2—CH(CH2—OH)—(O—CH2—CH(OH)—CH2)2—, —O—CH2—CH2—CH(CH2—OH)—(O—CH2—CH2—CH(OH)—CH2)2—, —O—CH2—CH(OH)—CH2—, —O—CH2—CH2—CH(OH)—CH2—CH2—, —O—CH2—CH(OH)—CH2—O—CH2—CH2—CH2—CH2—O—CH2—CH(OH)—CH2—, and —CO—NH—C(CH3)2—CO—;
R1 and R2 are each independently C1 to C6 alkyl optionally substituted with —OH;
R3 is C2 to C6 alkyl or C4 to C6 cycloalkyl;
L is O;
n is 1 or 2;
and
Ar is a phenyl or naphthyl group optionally substituted with up to 5:
C1 to C3 unsubstituted alkyl, C3 to C6 branched alkyl, unsubstituted aryl, or flourine groups;
or
a pyridal group optionally substituted with up to four alkyl groups.

10 more elements

A chromatography resin having the following formula:

Chromatography matrix-(X)—N(R1)—[(R3-L)n-Ar]2
or an anionic salt thereof,
wherein:
X is a spacer from the group consisting of —O—CH2—, —O—CH2—CH2—, —O—CH2—CH2—CH2—, —O—CH2—CH2—CH2—CH2—, —O—CH2—CH(CH2—OH)—(O—CH2—CH(OH)—CH2)2—, —O—CH2—CH2—CH(CH2—OH)—(O—CH2—CH2—CH(OH)—CH2)2—, —O—CH2—CH(OH)—CH2—, —O—CH2—CH2—CH(OH)—CH2—CH2—, —O—CH2—CH(OH)—CH2—O—CH2—CH2—CH2—CH2—O—CH2—CH(OH)—CH2—, and —CO—NH—C(CH3)2—CO—;
R1 is C1 to C6 alkyl optionally substituted with —OH;
R3 is C2 to C6 alkyl or C4 to C6 cycloalkyl;
L is O;
n is 1;
and
Ar is a phenyl or naphthyl group optionally substituted with up to 5:
C1 to C3 unsubstituted alkyl, C3 to C6 branched alkyl, unsubstituted aryl, or flourine groups;
or
a pyridal group optionally substituted with up to four alkyl groups.

10 more elements

Dependencies are inferred from the claim text.

Anionic exchange-hydrophobic mixed mode chromatography resin

Life of this patent

Anionic exchange-hydrophobic mixed mode chromatography resin

Life of this patent

Abstract

Claims14