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US 12,343,397 B2Granted
Drug-resistant immune cells and methods of use thereof
AssigneeRegents of the University of Minnesota
InventorsMark J. Osborn, Keli Hippen, Bruce R. Blazer
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Abstract
The present disclosure provides modified cells including pluripotent stem cells, hematopoietic precursor cells, and hematopoietic cells (e.g., modified Tregs) that are steroid and/or calcineurin inhibitor-resistant. The present disclosure provides methods for generating steroid and/or calcineurin inhibitor-resistant modified cells including pluripotent stem cells, hematopoietic precursor cells, and hematopoietic cells. Also provided herein are compositions and methods of treatment.
Claims13
A method for generating a modified cell, comprising:
- introducing a gene editing system into a regulatory T (Treg) cell that produces a modification in an exon, a splice donor, or a splice acceptor of a gene locus encoding for NR3C1, wherein the modification downregulates gene expression of NR3C1 and renders the modified cell steroid-resistant;
- and
- inserting an exogenous calcineurin inhibitor resistance gene into the genome of the cell to render the modified cell calcineurin inhibitor (CNI)-resistant,
- thereby generating a modified Treg cell.
1 more element
Dependencies are inferred from the claim text.