Wash for electrophoresis
AssigneeHelena Laboratories Corporation
InventorTipton L Golias
Life of this patent
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Abstract
A wash solution for removal of unbound proteins, unreacted antisera, and excess stain from an electrophoresis gel plate and a method of using the wash solution. The wash solution contains one or more of Hexamethylindanopyran, Tetramethyl acetyloctahydronaphthalenes, Hexyl cinnamal, Butylphenyl methylpropional, d-Limonene and Linalool. Alternatively, the wash solution react with lipids and fats in the unbound proteins and/or unreacted antisera, and/or causes pores in the gel to expand yielding a more efficient removal of unbound protein and unbound antisera, and/or contains protease enzymes that digest proteins and an amylase enzyme to hydrolyze starches and sugars.
Claims14
An improved wash solution for removing unbound proteins and unreacted antisera from a gel plate in an immunofixation (IFE) system, the wash solution including at least one of the components (a) through (f):
- a) Hexamethylindanopyran—C18H26O also known as 1,3,4,6,7,8-hexahydro-4,6,6,7,8,8,-hexamethyl-cyclopenta[g]benzopyran or HHCB;
- b) Tetramethyl acetyloctahydronaphthalenes—C16H26O;
- c) Hexyl cinnamal—C15H20O;
- d) Butylphenyl methylpropional—C14H20O;
- e) d-Limonene—C10H16;
- and
- f) 3,7-Dimethyl-3-hydroxy-1,6-octadiene—C10H18O.
Dependencies are inferred from the claim text.