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US 12,339,205 B2Granted
Method for recovering extracellular vesicles
AssigneeH.U. Group Research Institute G. K.
InventorsYuki Kawasaki, Ayako Kurimoto, Tatsutoshi Inuzuka
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Abstract
A method involves recovery an extracellular vesicle from an extracellular vesicle-containing sample. Such a method of recovering the extracellular vesicle may include separating the extracellular vesicle from the extracellular vesicle-containing sample in the presence of a polymer.
Claims19
A method of recovering an extracellular vesicle, the method comprising:
- separating the extracellular vesicle from an extracellular vesicle-containing sample in the presence of a polymer,
- wherein the separation is performed by an extracellular vesicle membrane-binding material comprising an antibody against an extracellular vesicle marker or an antigen-binding fragment thereof,
- wherein the polymer comprises carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, or hydroxypropylmethyl cellulose,
- wherein the antibody is against CD9, CD63, CD81, or CD147.
1 more element
A method of analyzing an extracellular vesicle, the method comprising:
- separating the extracellular vesicle from an extracellular vesicle-containing sample in the presence of a polymer, to obtain a separated extracellular vesicle;
- and
- analyzing the separated extracellular vesicle,
- wherein the separation is performed by an extracellular vesicle membrane-binding material comprising an antibody against an extracellular vesicle marker or an antigen-binding fragment thereof,
- wherein the polymer comprises carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, or hydroxypropylmethyl cellulose
- wherein the antibody is against CD9, CD63, CD81, or CD147.
3 more elements
A kit, comprising:
- a polymer;
- and
- an extracellular vesicle membrane-binding material,
- wherein the extracellular vesicle membrane-binding material comprises an antibody against an extracellular vesicle marker or an antigen-binding fragment thereof,
- wherein the polymer comprises carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, or hydroxypropylmethyl cellulose,
- wherein the antibody is against CD9, CD63, CD81, or CD147.
3 more elements
Dependencies are inferred from the claim text.