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US 12,338,470 B2Granted
Enzymes and systems for synthesizing DNA
AssigneeIridia, Inc.
InventorsPaul F. Predki, Stefen Boehme
Life of this patent
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Abstract
The disclosure provides novel topoisomerases which exhibit weakened ability to form a covalent bond to the 5′-(C/T)CCTT-3′recognition site in the presence of increased NaCl concentrations relative to wild-type, together with novel methods for synthesizing DNA in the 3′ to 5′ direction using the novel topoisomerase; and other compounds, compositions, methods and devices comprising or utilizing topoisomerases which exhibit reduced covalent bond formation to the 5′-(C/T)CCTT-3′ recognition site in the presence of increased NaCl concentrations, relative to wild-type.
Claims5
A topoisomerase comprising the following sequence:
- SEQ ID NO:
- 3
- RALFYKDGKLFTDNNFLNPVSDDNPAYEVLQHVKIPTHLTDVVVYEQTWE
- EALTRLIFVGSDSKGX1RX2X3FYGKMHVQNX4NAKRDRIFVRVYNVMKR
- INCFINKNIKKSSTDSNYQLAVFMLMETMFFIRFGKMKYLKENETVGLLT
- LKNKHIEISPDEIVIKFVGKDKVSHEFVVHKSNRLYKPLLKLTDDSSPEE
- FLFNKLSERKVYECIKQFGIRIKDLRTYGVNYTFLYNFWTNVKSISPLPS
- PKKLIALTIKQTAEVVGHTPSISKRAYMATTILEMVKDKNFLDVVSKTTF
- DEFLSIVVDHVKSSTDG
- wherein at least two of X1, X2, X3, and X4, are mutated from the residues in the wild type sequence [wherein in the wild-type sequence, X1 is arginine (R), X2 is glutamine (Q), X3 is tyrosine (Y), and X4 is arginine (R)] to glycine (G), alanine (A) or valine (V);
- provided that where only two residues are mutated, X1 and X2 are not both mutated to A.
8 more elements
Dependencies are inferred from the claim text.