Sucrose invertase variants

A sucrose invertase variant comprising a polypeptide sequence having at least 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% amino acid sequence identity to:

• (i) a full-length wild-type Saccharomyces cerevisiae sucrose invertase having the amino acid sequence of SEQ ID NO:
• 1 or (ii) SEQ ID NO:1 lacking the first 19 amino acids, wherein the sucrose invertase variant comprises a modification comprising at least one amino acid substitution in at least one amino acid residue position selected from the group consisting of positions 17, 28, 359, and 366, wherein the amino acid residue positions correspond to amino acid residues numbered from the N-terminus of SEQ ID NO:1, wherein the one or more amino acid substitutions are selected from the group consisting of:
• (a) an amino acid substitution from isoleucine to a hydroxyl- or sulfur-containing amino acid at amino acid residue position 17;
• (b) an amino acid substitution from arginine to an aliphatic or sulfur-containing amino acid at amino acid residue position 28;
• (c) an amino acid substitution from asparagine to a basic amino acid at amino acid residue position 359;
• and
• (d) an amino acid substitution from phenylalanine to a sulfur-containing or aliphatic amino acid at amino acid residue position 366.