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US 12,337,287 B2Granted
Microfluidic devices
AssigneeBio-Rad Laboratories, Inc.
InventorsDarren Roy Link, Michael Weiner, David Marran, Jonathan M. Rothberg
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Abstract
The present invention provides novel microfluidic substrates and methods that are useful for performing biological, chemical and diagnostic assays. The substrates can include a plurality of electrically addressable, channel bearing fluidic modules integrally arranged such that a continuous channel is provided for flow of immiscible fluids.
Claims20
A method for droplet formation for sample preparation for sequencing, the method comprising:
- providing, via at least one inlet channel on a microfluidic substrate, an aqueous sample fluid comprising nucleic acid and at least one primer;
- providing an immiscible first carrier fluid in a main channel on the microfluidic substrate;
- producing a plurality of droplets comprising the aqueous sample fluid at a junction between the main channel and the at least one inlet channel;
- copying at least a portion of the nucleic acid within one droplet of the plurality of droplets by extending the at least one primer to generate an extension product;
- and
- breaking the plurality of droplets by introducing a droplet-destabilizing second fluid comprising a destabilizing surfactant, thereby recovering the aqueous sample fluid including the extension product from the plurality of droplets.
3 more elements
A sample preparation method comprising:
- flowing, via an inlet channel on a microfluidic substrate, an aqueous sample fluid comprising nucleic acid to a junction between the inlet channel and a main channel having an immiscible carrier fluid therein;
- producing a plurality of droplets comprising the aqueous sample fluid surrounded by the immiscible carrier fluid in the main channel;
- copying at least a portion of the nucleic acid within one droplet of the plurality of droplets by extending a primer to generate an extension product;
- and
- recovering the aqueous sample fluid including the extension product from the plurality of droplets by introducing a droplet-destabilizing second fluid comprising a perfluorinated alcohol.
2 more elements
Dependencies are inferred from the claim text.