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US 12,336,551 B2Granted
Purified protein composition
AssigneeImpossible Foods Inc.
InventorsCarl Allen Henderson, Jr., Ratnayake Mudiyanselage Dunilka Nishani Ratnayake, Ian Guiles Ronningen, Laila Dafik
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Abstract
Described herein are methods for purifying protein, and more particularly to methods for purifying protein that minimize the development of undesirable odors and flavors in the purified protein and increase protein yield.
Claims20
A protein composition produced by a method comprising:
- a) lysing an aqueous suspension of a plurality of cells to obtain a cell lysate;
- and
- b) filtering the cell lysate to obtain a protein composition,
- wherein steps a) and b) independently, are performed at a pH between about 8.5 and about 12.0;
- and
- wherein:
- (i) the protein composition forms a gel upon heating to 65° C.;
- (ii) the protein composition forms a gel with a storage modulus of at least about 100 Pa when heated at or above about 85° C. for about 20 minutes;
- (iii) heating a 10% (w/v) suspension of the protein composition to at least about 95° C. results in a gel with a storage modulus of at least about 100 Pa;
- (iv) the protein composition forms a gel between about pH 5.5 and about pH 10.0, or
- (v) the protein composition forms a gel in solution with ionic strength below about 0.5 M, wherein the ionic strength is calculated based on the concentration of non-protein solutes.
8 more elements
Dependencies are inferred from the claim text.