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US 12,332,254 B2Granted
Systems, compositions, and methods for evaluating biomechanical properties of cells
AssigneeCornell University
InventorsNicholas Abbott, Karthik Nayani
Life of this patent
Estimating the term…
Abstract
The response of biological cells to applied stress is central to the functioning of living systems. The present disclosure is directed to systems, compositions and methods for rapidly reporting biomechanical properties of cells or cellular organelles at a single cell or population level.
Claims25
A system, comprising:
- a liquid crystal material, wherein the liquid crystal material has a first state at a first condition and optionally a second state at a second condition;
- a device for holding a cell or an organelle of a cell, and the liquid crystal material, wherein the device is designed for use in optical imaging the cell or the organelle in the presence of the liquid crystal material at an isotonic concentration;
- and
- wherein the system is configured to compare a first state of the cell or the organelle in the first state of the liquid crystal material to a second state of the cell or the organelle at the second state of the liquid crystal material or a second state in an aqueous buffer to determine a biomechanical property of the cell or the organelle.
1 more element
A composition, comprising a liquid crystal material at an isotonic concentration and a cell or an organelle of a cell,
- wherein the liquid crystal material has a first state at a first temperature or a first concentration and a second state at a second temperature or a second concentration, and
- wherein the cell or the organelle has a first state of the cell or the organelle in the first state of the liquid crystal material and a second state of the cell or the organelle at the second state of the liquid crystal material,
- such that the composition having the first state and the second state of the cell or the organelle in the liquid crystal material are configured to determine a biomechanical property of the cell or the organelle.
A method of evaluating a cell or an organelle of a cell, comprising:
- detecting a first state of the cell or the organelle in an aqueous buffer;
- detecting a second state of the cell or the organelle in a liquid crystal material;
- comparing the first state of the cell or the organelle to the second state of the cell or the organelle, thereby determining a biomechanical property of the cell.
A method of evaluating a cell or an organelle of a cell, comprising
- placing the cell or the organelle in a liquid crystal material at a first state, and detecting a first state of the cell or the organelle;
- effecting a change in the liquid crystal material from the first state to a second state, and detecting a second state of the cell or the organelle after the change;
- wherein the first state of the liquid crystal material is less ordered than the second state of the liquid crystal material, or the first state of the liquid crystal material is more ordered than the second state of the liquid crystal material;
- and
- comparing the first state of the cell or the organelle to the second state of cell or the organelle, thereby determining a biomechanical property of the cell or the organelle.
2 more elements
A method of evaluating cells or organelles, comprising:
- placing the cells or the organelles in a liquid medium, wherein a part of the liquid medium is in a liquid crystal state, and another part of the liquid medium is in an isotropic state, and wherein a first group of cells or organelles are in the part of the liquid medium that is in the liquid crystal state, and a second group of cells or organelles are in the part of the liquid medium that is in the isotropic state;
- detecting a state of the first group of cells or organelles and a state of the second group of cells or organelles;
- and
- comparing the state of the first group of cells or organelles to the state of the second group of cells or organelles, thereby determining a biomechanical property of the cells or the organelles.
1 more element
Dependencies are inferred from the claim text.