Apparatus, system, and method for testing biological samples

A method of testing a biological sample, the method comprising:

• a) providing an apparatus, the apparatus comprising:
• a frame comprising:
• a first side,
• a second side,
• a third side,
• a fourth side, the third side having a first longitudinal passageway and the fourth side having a second longitudinal passageway, the first and second longitudinal passageways configured as fluid conduits, the frame comprising no other fluid conduits other than the first and second longitudinal passageways, the first side, the second side, the third side, and the fourth side forming an outer surface of the frame;
• and
• an aperture extending from the first side to the second side, the first and second longitudinal passageways extending through the frame to the aperture;
• a first cover attached to the first side and covering the aperture;
• a second cover attached to the second side and covering the aperture;
• an electromagnetic imaging device configured to image the biological sample through at least one of:
• the first cover, or the second cover;
• a well formed by the frame, the first cover, and the second cover;
• a first tube fluidly coupled to the well via the first longitudinal passageway;
• and
• a second tube fluidly coupled to the well via the second longitudinal passageway, the first and second covers forming a fluid tight and leak-free joint with the frame such that no fluid escapes from the well except via the first tube and the second tube;
• b) supplying a first fluid to the well via the first tube, the first fluid comprising live cells;
• c) growing the biological sample in the form of a biofilm in the well from the live cells in the first fluid;
• d) supplying a second fluid to the well via the first tube, the second fluid being sterile;
• e) imaging the biofilm within the well, via the electromagnetic imaging device through at least one of:
• the first cover, or the second cover;
• wherein a depth of the well as defined by a distance between the first cover and the second cover is greater than at least one of a diameter of the first tube or a diameter of the second tube.

A system for testing one or more biological samples, the system comprising:

• a frame having a first side, a second side, a plurality of apertures extending from the first side to the second side, and a third side having a first longitudinal passageway extending through the frame to one of the plurality of apertures, the first side, the second side, the third side, and the fourth side forming an outer surface of the frame;
• a plurality of first covers, each of the plurality of first covers being configured to be attached to the first side for covering one of the plurality of apertures;
• and a plurality of second covers, each of the plurality of second covers being configured to be attached to the second side for covering one of the plurality of apertures;
• wherein when attached to the frame, a distance between the plurality of first covers and the plurality of second covers is greater than a diameter of the first longitudinal passageway;
• and wherein at least one of the plurality of first covers or the plurality of second covers is configured to allow an electromagnetic imaging device to image one or more biological samples.