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US 12,331,352 B2Granted
DNA sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
AssigneeThe Trustees of Columbia University in the City of New York
InventorsJingyue Ju, Dae Hyun Kim, Jia Guo, Qinglin Meng, Zengmin Li, Huanyan Cao
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Abstract
This invention provides a process for sequencing nucleic acids using 3′ modified deoxynucleotide analogues or 3′ modified deoxyinosine triphosphate analogues, and 3′ modified dideoxynucleotide analogues having a detectable marker attached thereto.
Claims20
A deoxyribonucleic acid having attached at a 3′ end thereof, by a phosphodiester bond, a compound having the structure:
- wherein the α labeled O atom is the 3′ O atom of the deoxyribonucleic acid, the wavy line represents the remainder of the deoxyribonucleic acid that is 5′ relative to the 3′O, and wherein R′ is a cleavable chemical group.
Dependencies are inferred from the claim text.