Back to results
US 12,331,341 B2Granted
Serum-free cell culture medium
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsShadia Abike Oshodi, Amy S. Johnson, Shawn M. Lawrence
Life of this patent
Estimating the term…
Abstract
The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.
Claims14
A method for cultivating CHO cells, comprising the steps of:
- (a) providing a cell culture medium which is serum-free, comprising 0.6±0.09 mM ornithine and 0.714±0.11 mM putrescine, and (b) propagating or maintaining the CHO cells in the cell culture medium to form a cell culture, wherein the cells express aflibercept, wherein the cell culture has a greater viable cell culture density than an equivalent aflibercept-expressing CHO cell culture grown in a cell culture medium that is supplemented with no more than 2.5 μM putrescine and no ornithine.
Dependencies are inferred from the claim text.