Carboxyesterase biocatalysts
AssigneeGlaxoSmithKline Intellectual Property Development Limited
InventorsKristin K. Brown, Brent M. Dorr, Douglas E. Fuerst, Katherine Joyce Honicker, Lydia Sanchez Jordan, James Patrick Morrison, Nikolay V. Plotnikov, Markus Schober, Rama Voladri
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Abstract
The present disclosure provides engineered carboxyesterase enzymes that have the ability to catalyze amide bond formation. Also provided are polynucleotides encoding the carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes to make commercially valuable amides. Also provided are amides that are made using the engineered carboxyesterase enzymes.
Claims15
A carboxyesterase polypeptide comprising an amino acid sequence that is at least 90% identical to the amino acid sequence set forth in SEQ ID NO:
- 4, wherein the residue corresponding to X198 in SEQ ID NO:
- 4 is a phenylalanine (F) residue, an isoleucine (I) residue, a tyrosine (Y) residue, a leucine (L) residue, or a methionine (M) residue.
Dependencies are inferred from the claim text.