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US 12,331,314 B2Granted
Culture method for organoids
AssigneeKoninklijke Nederlandse Akademie van Wetenschappen
InventorsMeritxell Huch Ortega, Johannes Carolus Clevers, Sylvia Fernandez Boj
Life of this patent
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Abstract
The invention relates to improved culture methods for expanding epithelial stem cells and obtaining organoids, to culture media involved in said methods, and to uses of said organoids.
Claims15
A differentiation medium comprising a basal medium for animal or human cells to which is added one or more receptor tyrosine kinase ligands, a Notch inhibitor, a compound that induces the phosphorylation of SMAD1 or SMAD5, and a TGF-beta inhibitor.
Dependencies are inferred from the claim text.